Post-Doc: Design and development of gene delivery systems for oral route

Supervisors: Dr. Giovanna Lollo

Keywords: Nanocarriers, nucleic acid, physicochemical characterization, oral administration, in vivo studies


Starting date: January 2022 (1218 months)


Laboratory: The LAGEPP (https://lagepp.univlyon1.fr/en/home/) is a research laboratory of the University Lyon 1 and the French national Institute for scientific research (CNRS UMR 5007). It develops pluridisciplinary research in chemical engineering, pharmaceutical engineering and biochemical processes. The Gepharm “pharmaceutical engineering” group has a strong expertise in drug delivery. The research encompasses several scientific domains as development of new drug delivery systems, physical chemistry properties, biological in vitro evaluation, in vivo biodistribution and efficacy studies in mice models.


The postdoc will be part of the ANR project HyDNano working closely with different partners from industry and academic research groups. The aim of the project is to optimize and characterize novel nanomedicines
for the oral delivery of genetic material for autoimmune disease.


Principal activities:

The post doc will be involved in the:

i) optimization of nanoparticulate systems based on either FDA approved materials or new chemical entities to ensure the correct biodistribution of the systems in vivo;

ii) evaluation of the association, integrity and activity of the encapsulated/associated genetic material;

iii) development of in vitro and in vivo tests to validate the efficacy of the selected systems. An extensive characterization of immune responses is envisaged to provide a better understanding of immune landscape
involved in the healthy and inflamed tissues.

iv) in vivo imaging to study nanoparticles biodistribution, ex vivo flow cytometry, microscopy and image data analysis will be also performed.


Required skills:

Scientific:

Previous experience in:

Design and development of nanomedicines for gene delivery (experience with microfluidic is a plus)

Evaluation of nucleic acid association to the nanomedicines (electrophoresis, ITC, NTA)

in vitro cell culture studies (viability MTTMTS, FACS analysis, confocal microscopy, Western Blot)

in vivo animal experience

Soft skill:

Motivated for the position

Enthusiastic, ambitious and strong commitment to research

Excellent communication, presentation and writing skills

Able to work independently and collaborate in team

Good sense for initiative and quality, accuracy and detail, team player.

Language: English is required. French is a plus.

The application must include:

CV

Motivation Letter

PhD degree diploma

Two reference letters

Academic works published or unpublished that you would like to be considered in the assessment (up to 3 works)



Contacts :
giovanna.lollo@univlyon1.fr; stephanie.briancon@univlyon1.fr

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