Thesis defense: Malgorzata Tarnowska

Malgorzata Tarnowska will support her PhD on December 3 at 1400 in BU University’s Doua conference room

Title : Evaluation of skin absorption of inorganic ions with regard to their physicochemical properties
Jury :
Majella LANE, Senior Lecturer (University College London) Rapporteur
Cécile LAUGEL, Professeur (Université Paris Sud) Rapporteur
Yves CHEVALIER, Directeur de Recherche CNRS (Université de Lyon) Examinateur
Philippe LAWTON, Professeur (Université de Lyon) Examinateur
Emmanuelle MARIE-BEGUE, Chargé de Recherche CNRS (ENS, Paris) Examinatrice
Thierry POURCHER, Directeur de Recherche CEA (Université Côte d’Azur) Examinateur
Marie-Alexandrine BOLZINGER, Professeur (Université de Lyon) Directrice de thèse
Stéphanie BRIANCON, Professeur (Université de Lyon) Co-directrice de thèse

Summary: Human skin forms a unique interface between the body and the external environment. Its main role is to protect the internal organs from external factors. Its highly hydrophobic outermost layer, stratum corneum, has long been believed impermeable for highly hydrophilic compounds, including ions. Several studies proved this concept wrong, and recent research by Paweloszek et al. demonstrated the important contribution of facilitated transport in permeation of halide anions.
Skin penetration of anions classified in Hofmeister series (of F-, Br-, I-, SCN, ClO4-) alone and in bi- and ternary mixtures in two experimental series was studied in vitro. All tested ions permeated viable skin within 24h. Among halides, the presence of F- reduced the penetration of Br- and I- in mixtures, and synergy between Br- and I- was observed. Within the second group (I-, SCN-, ClO4-) the inhibition of ClO4- penetration in the presence of other ions was observed.
Finally, the impact of formulation of marketed thermal spring water (TSW) into emulsions (TSW/O, O/TSW, TSW/O/W) and liposomes on skin absorption of Ca2+ and Mg2+ was evaluated. Liposomes and emulsions promoted retention of Ca2+ and Mg2+ in skin layers as compared to TSW. Our results prove that the beneficial effects observed during treatment with TSW are associated with penetration of the minerals into and through the skin and are not only a surface action.
In this thesis, we demonstrate the possibility of both anions and cations to penetrate viable skin in vitro, and we disclose the effects of mixing and formulating on skin penetration profiles.
Keywords: skin penetration, inorganic ions, Hofmeister series, Thermal Spring Waters, formulation

Date(s) - 3 Dec 2019
14 h 00 min - 16 h 00 min


Filed under: Defense