Greta Camilla MAGNANO’s Phd Defense

Titre :  “Design of nanoparticles for skin decontamination against chemical warfare agents. Study of the physicochemical properties influencing degradation and formulation processes”

Date : le 31 janvier 2020 à 10h30  amphi de la BU de la Doua

Jury members :

– Amélie BOCHOT, PU/ Fac de Pharmacie de Châtenay-Malabry  (Rapporteur)

– Frédéric DORANDEU,   – Prof Agrégé/ DGA/IRBA Brétigny-sur-Orge (Rapporteur)

– Thierry GACOIN –  DR /Ecole Polytechnique de Paris  (Rapporteur)

– Stéphanie BRIANCON, PU /Univ Lyon 1 (Directrice de thèse)

-Thierry DEVERS – MDC/ Université d’Orléans (examinateur)

– Christian JALLUT – PU/ Univ Lyon 1 (examinateur)

– Jennifer MILLERIOUX – DGA/Vers-le-Petit (examinatrice)

– Marie-Alexandrine BOLZINGER – PU/Univ Lyon 1 (invité)

– Denis JOSSE – Pharmacien Chef/CBRNE (invité)

– David Amans – MDC/ Univ Lyon 1 (invité)

ABSTRACT : The exposure to Organophosphorus compounds (OPs) during terrorist attacks and military conflicts is a severe threat. The OPs belong to the CWA group, and enter the body mainly through ingestion and skin contact, rapidly inducing general intoxication and even death of the exposed person.

A rapid skin decontamination is the most efficient way to reduce the risk of intoxication. The conventional procedure, including application of absorbent powders and water or active solutions rinsing, presents some limitations.  Cerium dioxide nanoparticles (CeO2NPs) arepromising systems due to their high absorbent properties related to their specific surface and their surface activity ensuring a rapid degradation of the toxic agent. This work was dedicated to the study of degradation mechanisms of Paraoxon (POX), a model OPs, using CeO2NPs. The results clearly showed the influence of two physico-chemical parameters of NPs on degradation kinetics of the toxic, the shape and the crystal faces. In particular, the crystalline facets {111} are found more favorable for the degradation of POX than the faces {100}. The nano octahedra, which is rich in {111} planes, was the most effective morphology, allowing a rapid and total degradation of POX.  Then,decontamination systems were designed using CeO2 NPs inthe form of powder or pastes and their efficiency were evaluated in vitro – ex vivoin a Franz diffusion cell using pig ear skin. The results showed that all the systems significantly reduced the contamination compared to non decontaminated control. The decontamination efficacy of systems containing water was higher than that of powders.

Keywords : Paraoxon, CeO2NPs, skin decontamination systems, degradation mechanism, morphology of NPs

Date(s) - 31 Jan 2020
10 h 30 min - 13 h 30 min

Bibliothèque de la Doua


Filed under: Defense